TSFM associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome. TSFM localizes in mitochondrion.1) Vernon, J.L., et al., (2000) Cytogenet. Cell Genet. 89 (3-4), 145-146.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TSFM.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
TSFM antibody can be used for detection of TSFM by ELISA at 1:312500. TSFM antibody can be used for detection of TSFM by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
TSFM antibody can be used for detection of TSFM by ELISA at 1:312500. TSFM antibody can be used for detection of TSFM by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store TSFM antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt