Application
Western Blot at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis in CEM,K562,HL-60 cell line lysates (35ug/lane).Application
Flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TSPAN2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tetraspanin-2, Tspan-2, Tetraspan NET-3, TSPAN2 |
| Product description | TSPAN2 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility.1) Davila,S., et.al., Genes Immun. 11 (3), 232-238 (2010) |
| Immunogen | This TSPAN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-137 amino acids from the Central region of human TSPAN2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TSPAN2 |
|---|---|
| Alternative Names | Tetraspanin-2 Tspan-2 Tetraspan NET-3 TSPAN2 |
| Molecular Weight(MW) | 24 kDa |
Application
Western Blot at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis in CEM,K562,HL-60 cell line lysates (35ug/lane).
Application
Flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
