TUBB2A belongs to the tubulin family. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.1) Frum, R., (2007) J. Proteome Res. 6 (4), 1410-1417.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TUBB2A.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
TUBB2A antibody can be used for detection of TUBB2A by ELISA at 1:62500. TUBB2A antibody can be used for detection of TUBB2A by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.
Positive Control
1) 721_B Cell Lysate
Application Notes
TUBB2A antibody can be used for detection of TUBB2A by ELISA at 1:62500. TUBB2A antibody can be used for detection of TUBB2A by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store TUBB2A antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.