Application
Western Blot at 1:1000 dilution Lane 1: human brain lysate Lane 2: human cerebellum lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis in mouse brain tissue lysates (15ug/lane).Application
Western blot analysis in HepG2 cell line lysates (15ug/lane).Application
TUBB3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TUBB3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III, TUBB3, TUBB4 |
| Product description | Beta III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of beta III is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells.1) Khan, I.A., et al. Biochemistry 35(12):3704-3711(1996) |
| Immunogen | This TUBB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-63 amino acids from the N-terminal region of human TUBB3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TUBB3 |
|---|---|
| Alternative Names | Tubulin beta-3 chain Tubulin beta-4 chain Tubulin beta-III TUBB3 TUBB4 |
| Molecular Weight(MW) | 50 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Western Blot at 1:1000 dilution Lane 1: human brain lysate Lane 2: human cerebellum lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis in mouse brain tissue lysates (15ug/lane).
Application
Western blot analysis in HepG2 cell line lysates (15ug/lane).
Application
TUBB3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
