Application
Western blot analysis of lysed extracts from JK, Raji and HL-60 cells using Twist1 Antibody.| Product Name | Twist1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CRS, SCS, ACS3, CRS1, BPES2, BPES3, TWIST, bHLHa38 |
| Product description | Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation.1) "Twist regulates cytokine gene expression through a negative feedback loop that represses NF-kappaB activity. "Sosic D., Richardson J. A., Yu K., Ornitz D. M., Olson E. N. Cell 112:169-180 (2003) "Mutations of the TWIST gene in the Saethre-Chotzen syndrome. "el Ghouzzi V., le Merrer M., Perrin-Schmitt F., et al., Nat. Genet. 15:42-46 (1997) |
| Immunogen | Twist1 antibody was raised against a peptide sequence around aa.18~22 (S-N-S-E-E) derived from Human Twist1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TWIST1 |
|---|---|
| Alternative Names | CRS SCS ACS3 CRS1 BPES2 BPES3 TWIST bHLHa38 |
| Molecular Weight(MW) | 28 kDa |
| Tissue Specificity | This antibody detects endogenous levels of total Twist1 protein. |
| Entrez Gene | 7291 |
|---|---|
| Protein Accession | NP_000465.1 |
Application
Western blot analysis of lysed extracts from JK, Raji and HL-60 cells using Twist1 Antibody.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
