Application
Western blot analysis of anti-YARS Antibody in CEM cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with YARS antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | Tyrosyl tRNA synthetase Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tyrosine--tRNA ligase, cytoplasmic, Tyrosyl-tRNA synthetase, TyrRS, Tyrosine--tRNA ligase, cytoplasmic, N-terminally processed, YARS |
| Product description | Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine.1) Yang,X.L., Chem. Biol. 14 (12), 1323-1333 (2007) |
| Immunogen | This Tyrosyl tRNA synthetase (YARS) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 450-479 amino acids from the C-terminal region of human Tyrosyl tRNA synthetase (YARS). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | YARS |
|---|---|
| Alternative Names | Tyrosine--tRNA ligase cytoplasmic Tyrosyl-tRNA synthetase TyrRS Tyrosine--tRNA ligase cytoplasmic N-terminally processed YARS |
| Molecular Weight(MW) | 59 kDa |
Application
Western blot analysis of anti-YARS Antibody in CEM cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with YARS antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
