Application
Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.| Product Name | U2AF2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | U2AF2, U2AF65 |
| Product description | U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2AF2 is the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly.U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.1) Sickmier, E.A., Acta Crystallograph. Sect. F Struct. Biol. Cryst. Commun. 62 (PT. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human U2AF2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| U2AF2 antibody can be used for detection of U2AF2 by ELISA at 1:62500. U2AF2 antibody can be used for detection of U2AF2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | U2AF2 |
|---|---|
| Alternative Names | U2AF2 U2AF65 |
| Molecular Weight(MW) | 52 kDa, 54 kDa |
| Entrez Gene | 11338 |
|---|---|
| Protein Accession | NP_001012496 |
Application
Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | U2AF2 antibody can be used for detection of U2AF2 by ELISA at 1:62500. U2AF2 antibody can be used for detection of U2AF2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store U2AF2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
