Application
Western blot analysis of UBAP2L in HeLa cell lysate with UBAP2L antibody at 1 μg/ml.Application
Immunofluorescence of UBAP2L in HeLa cells with UBAP2L antibody at 20 μg/ml.Application
Immunocytochemistry of UBAP2L in HeLa cells with UBAP2L antibody at 10 μg/ml.| Product Name | UBAP2L Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ubiquitin associated protein 2-like, NICE-4 |
| Product description | The UBA domain-containing protein ubiquitin associated protein 2-like (UBAP2L), also known as NICE4, is a ubiquitously expressed 1,087 amino acid protein. UBAP2L is preferentially expressed in hematopoietic stem cell (HSC) populations and interacts with BMI1, a well-known determinant of HSC function (1). It is essential for the activity of long-term repopulating HSCs but not the BMI1-dependent repression of the Ink4a/Arf locus (1).1) Bordeleau ME, Aucagne R, Chagraoui J, et al. UBAPL2L is a novel BMI1-iteracting protein essential for hematopoietic stem cell activity. Blood 2014; 124:2362-9. |
| Immunogen | UBAP2L antibody was raised against a 19 amino acid peptide from near the amino terminus of human UBAP2L. The immunogen is located within amino acids 200 - 250 of UBAP2L. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF |
| UBAP2L antibody can be used for the detection of UBAP2L by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry at 10 μg/ml.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UBAP2L |
|---|---|
| Alternative Names | Ubiquitin associated protein 2-like NICE-4 |
| Molecular Weight(MW) | Predicted: 120 kDa Observed: 130 kDa |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | UBAP2L antibody is human and mouse reactive. At least four isoforms of UBAP2L are known to exist; this antibody will detect all four isoforms. This antibody is predicted to not cross-react with UBAP2. |
Application
Western blot analysis of UBAP2L in HeLa cell lysate with UBAP2L antibody at 1 μg/ml.
Application
Immunofluorescence of UBAP2L in HeLa cells with UBAP2L antibody at 20 μg/ml.
Application
Immunocytochemistry of UBAP2L in HeLa cells with UBAP2L antibody at 10 μg/ml.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | UBAP2L antibody can be used for the detection of UBAP2L by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry at 10 μg/ml.: |
| Form | Liquid |
|---|---|
| Storage Instructions | UBAP2L antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | UBAP2L Antibody is supplied in PBS containing 0.02% sodium azide. |
