Application
Western blot analysis of lysate from Hela cell line, using UBE2D2 Antibody at 1:1000 at each lane.| Product Name | UBE2D2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ubiquitin-conjugating enzyme E2 D2, Ubiquitin carrier protein D2, Ubiquitin-conjugating enzyme E2(17)KB 2, Ubiquitin-conjugating enzyme E2-17 kDa 2, Ubiquitin-protein ligase D2, p53-regulated ubiquitin-conjugating enzyme 1, UBE2D2, PUBC1, UBC4, UBC5B, UBCH |
| Product description | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.1) Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) |
| Immunogen | This UBE2D2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-140 amino acids from the C-terminal region of human UBE2D2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UBE2D2 |
|---|---|
| Alternative Names | Ubiquitin-conjugating enzyme E2 D2 Ubiquitin carrier protein D2 Ubiquitin-conjugating enzyme E2(17)KB 2 Ubiquitin-conjugating enzyme E2-17 kDa 2 Ubiquitin-protein ligase D2 p53-regulated ubiquitin-conjugating enzyme 1 UBE2D2 PUBC1 UBC4 UBC5B UBCH |
| Molecular Weight(MW) | 17 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis of lysate from Hela cell line, using UBE2D2 Antibody at 1:1000 at each lane.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
