Application
Western Blot at 1:1000 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Formalin-fixed and paraffin-embedded breast carcinoma tissue reacted with UBE2L6 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | UBE2L6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ubiquitin/ISG15-conjugating enzyme E2 L6, Retinoic acid-induced gene B protein, RIG-B, UbcH8, Ubiquitin carrier protein L6, Ubiquitin-protein ligase L6, UBE2L6, UBCH8 |
| Product description | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBE2L6 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to function in the E6/E6-ap-induced ubiquitination of p53.1) Kumar, S., et al., J. Biol. Chem. 272(21):13548-13554 (1997). Ardley, H.C., et al., Cytogenet. Cell Genet. 89 (1-2), 137-140 (2000). |
| Immunogen | This UBE2L6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 123-153 amino acids from the C-terminal region of human UBE2L6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | UBE2L6 |
|---|---|
| Alternative Names | Ubiquitin/ISG15-conjugating enzyme E2 L6 Retinoic acid-induced gene B protein RIG-B UbcH8 Ubiquitin carrier protein L6 Ubiquitin-protein ligase L6 UBE2L6 UBCH8 |
| Molecular Weight(MW) | 18 kDa |
Application
Western Blot at 1:1000 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Formalin-fixed and paraffin-embedded breast carcinoma tissue reacted with UBE2L6 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
