Application
Western blot analysis of UBTD1 using rabbit polyclonal polyclonal antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UBTD1 gene (Lane 2).Application
Western blot analysis of in Hl60 cell line lysates (35ug/lane).Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | UBTD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ubiquitin domain-containing protein 1, UBTD1 |
| Product description | UBTD1 contains a ubiquitin domain, and has been demonstrated to bind to p53 in cells p53 is linked to the ubiquitination pathway through its transcriptional regulation of the four ubiquitin ligases, MDM2, COP1, Siah1b, and Pirh2. The role of UBTD1 has not been elucidated, however preliminary evidence indicates a potential role in p53 signaling.1) Ota, T., et al., Nat. Genet. 36(1):40-45 (2004). |
| Immunogen | This UBTD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 180-212 amino acids from the C-terminal region of human UBTD1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | UBTD1 |
|---|---|
| Alternative Names | Ubiquitin domain-containing protein 1 UBTD1 |
| Molecular Weight(MW) | 26 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis of UBTD1 using rabbit polyclonal polyclonal antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UBTD1 gene (Lane 2).
Application
Western blot analysis of in Hl60 cell line lysates (35ug/lane).
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
