Application
Western blot analysis of lysates from 293, 293T, SW620, U-87 MG cell line (from left to right), using UCHL3 Antibody (C209) at 1:1000 at each lane.Application
Western blot analysis of UCHL3 in HepG2 cell line lysates (35ug/lane)Application
Western blot analysis of his tag antibody in UCHL3 gene transfected 293 lysates (35ug/lane). His-tag UCHL3 protein is detected.Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with UCHL3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with UCHL3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | UCHL3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ubiquitin carboxyl-terminal hydrolase isozyme L3, UCH-L3, Ubiquitin thioesterase L3, UCHL3 |
| Product description | Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs). Mayer and Wilkinson (1989) identified 4 distinct UCH activities from bovine thymus. All 4 were thiol proteases and had high-affinity binding sites for ubiquitin. Wilkinson et al. (1989) purified the predominant isozyme, UCHL3, and raised antibodies against it. By screening a human B-cell expression library with the antibodies, the authors isolated cDNAs encoding human UCHL3. Sequence comparisons revealed that the sequence of the predicted 230-amino acid human UCHL3 protein is 54% identical to that of UCHL1.1) Saito, S., et al., J. Hum. Genet. 48(5):249-270 (2003). Wilkinson, K.D., et al., Science 246(4930):670-673 (1989). |
| Immunogen | This UCHL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-225 amino acids from the C-terminal region of human UCHL3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | UCHL3 |
|---|---|
| Alternative Names | Ubiquitin carboxyl-terminal hydrolase isozyme L3 UCH-L3 Ubiquitin thioesterase L3 UCHL3 |
| Molecular Weight(MW) | 26 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis of lysates from 293, 293T, SW620, U-87 MG cell line (from left to right), using UCHL3 Antibody (C209) at 1:1000 at each lane.
Application
Western blot analysis of UCHL3 in HepG2 cell line lysates (35ug/lane)
Application
Western blot analysis of his tag antibody in UCHL3 gene transfected 293 lysates (35ug/lane). His-tag UCHL3 protein is detected.
Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with UCHL3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with UCHL3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
