Application
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Antibody is used in Western blot to detect UCK in HepG2 cell lysate (Lane 1) and mouse cerebellum tissue lysate (Lane 2).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | UCK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UMP-CMP kinase {ECO:0000255|HAMAP-Rule:MF_03172}, 27414 {ECO:0000255|HAMAP-Rule:MF_03172}, Deoxycytidylate kinase {ECO:0000255|HAMAP-Rule:MF_03172}, CK {ECO:0000255|HAMAP-Rule:MF_03172}, dCMP kinase {ECO:0000255|HAMAP-Rule:MF_03172}, Nucleoside-diphosphate |
| Product description | UCK1 (Uridine-cytidine kinase 1) phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. This enzyme does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Of note, UCK1 is able to use either ATP or GTP as a phosphate donor. UCK1 also possesses the ability to phosphorylate a number of cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4-thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)-benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5-methylcytidine, and N(4)-anisoylcytidine.1) Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). |
| Immunogen | This UCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 132-162 amino acids from the C-terminal region of human UCK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CMPK1 |
|---|---|
| Alternative Names | UMP-CMP kinase {ECO:0000255|HAMAP-Rule:MF_03172} 27414 {ECO:0000255|HAMAP-Rule:MF_03172} Deoxycytidylate kinase {ECO:0000255|HAMAP-Rule:MF_03172} CK {ECO:0000255|HAMAP-Rule:MF_03172} dCMP kinase {ECO:0000255|HAMAP-Rule:MF_03172} Nucleoside-diphosphate |
| Molecular Weight(MW) | 22 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Antibody is used in Western blot to detect UCK in HepG2 cell lysate (Lane 1) and mouse cerebellum tissue lysate (Lane 2).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
