Application
Western blot analysis of UCP1 in human brain tissue lysate with UCP1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunohistochemistry of UCP1 in human liver tissue with UCP1 antibody at 2.5 μg/mL.| Product Name | UCP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UCP1 Antibody: UCP, SLC25A7, UCP, Mitochondrial brown fat uncoupling protein 1, Solute carrier family 25 member 7, UCP 1 |
| Product description | The mitochondrial brown fat uncoupling protein 1 (UCP1) is a member of the family of mitochondrial anion carrier proteins (MACP) (1). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells (1). UCP1 is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat (1).1) 1. Rial E, Gonzalez-Barroso MM, Fleury C, et al. The structure and function of the brown fat uncoupling protein UCP1: current status. Biofactors 1998; 8:209-19. |
| Immunogen | UCP1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human UCP1 (GenBank accession. The immunogen is located within the last 50 amino acids of UCP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| UCP1 antibody can be used for detection of UCP1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 2.5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UCP1 |
|---|---|
| Alternative Names | UCP1 Antibody: UCP SLC25A7 UCP Mitochondrial brown fat uncoupling protein 1 Solute carrier family 25 member 7 UCP 1 |
| Molecular Weight(MW) | Predicted: 34 kDa Observed: 35 kDa |
| Sequence Similarities | 2) Cat. No. 10-201 - Human Liver Tissue Slide |
| Tissue Specificity | UCP1 antibody is human, mouse and rat reactive. |
Application
Western blot analysis of UCP1 in human brain tissue lysate with UCP1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunohistochemistry of UCP1 in human liver tissue with UCP1 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | UCP1 antibody can be used for detection of UCP1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | UCP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | UCP1 antibody is supplied in PBS containing 0.02% sodium azide. |
