Application
Antibody used in WB on Human Liver at 2.5 ug/ml.| Product Name | UGT1A1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UGT1A1, GNT1, HUG-BR1, UDPGT, UGT1, UGT1A, BILIQTL1, UDPGT 1-1 |
| Product description | UGT1A1 is a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. The preferred substrate of this enzyme is bilirubin, although it also has moderate activity with simple phenols, flavones, and C18 steroids.This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The preferred substrate of this enzyme is bilirubin, although it also has moderate activity with simple phenols, flavones, and C18 steroids. Mutations in this gene result in Crigler-Najjar syndromes types I and II and in Gilbert syndrome.1) Wasmuth, H.E., (2006) Hepatology 43 (4), 738-741. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UGT1A1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| UGT1A1 antibody can be used for detection of UGT1A1 by ELISA at 1:1562500. UGT1A1 antibody can be used for detection of UGT1A1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | UGT1A1 |
|---|---|
| Alternative Names | UGT1A1 GNT1 HUG-BR1 UDPGT UGT1 UGT1A BILIQTL1 UDPGT 1-1 |
| Molecular Weight(MW) | 57 kDa |
Application
Antibody used in WB on Human Liver at 2.5 ug/ml.| Positive Control | 1) Cat. No. XBL-10409 - Fetal Liver Tissue Lysate |
|---|---|
| Application Notes | UGT1A1 antibody can be used for detection of UGT1A1 by ELISA at 1:1562500. UGT1A1 antibody can be used for detection of UGT1A1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store UGT1A1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
Data sheet for OM294611