Application
Western blot analysis of ULK2 in human brain tissue lysate with ULK2 antibody at 1 μg/mL.Application
Immunofluorescence of ULK2 in human brain tissue with ULK2 antibody at 20 μg/ml.Application
Immunohistochemistry of ULK2 in human brain tissue with ULK2 antibody at 2.5 μg/ml.WB
(A, B) ULK2 and GABARAPL1 expression decreased in OC tissues, compared to the paired paracancerous tissues specimens from 8 patients, determined by qRT-PCR analysis and western blot analysis.IHC
(C) Representative immunohistochemical images of ULK2 and GABARAPL1 expression in OC tissues and paracancerous tissues analyzed by IHC (400×).| Product Name | ULK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ULK2 Antibody: ULK2, also known as ATG1B, is a key serine/threonine protein kinase probably acting at the most upstream step of autophagosome formation. Knockout of ULK2 results in a severe defect in the autophagy pathway. ULK2 is highly conserved among eukaryotes and shows high homology with its related protein ULK1. Both ULK1 and ULK2 form a complex with ATG13 and FIP200 that mediates TOR signaling and is essential for autophagy. Like ULK1, ULK2 is also thought to be involved in early neuronal growth and differentiation.1) Suzuki K, Kubota Y, Sekito T, et al. Hierarchy of Atg proteins in pre-autophagosomal structure organization. Genes to Cells 2007; 12:209–18. |
| Immunogen | ULK2 antibody was raised against a 17 amino acid peptide near the center of human ULK2 . The immunogen is located within amino acids 480 - 530 of ULK2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIHC-PWB |
| ULK2 Antibody can be used for detection of ULK2 by Western blot at 1 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ULK2 |
|---|---|
| Alternative Names | ULK2 Antibody: ATG1B Unc51.2 KIAA0623 Unc-51-like kinase 2 |
| Molecular Weight(MW) | Predicted: 105, 114 kDa Observed: 100 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | At least two isoforms of ULK2 are known to exist; this antibody will detect both isoforms. ULK2 antibody is predicted to not cross-react with ULK1. |
Application
Western blot analysis of ULK2 in human brain tissue lysate with ULK2 antibody at 1 μg/mL.
Application
Immunofluorescence of ULK2 in human brain tissue with ULK2 antibody at 20 μg/ml.
Application
Immunohistochemistry of ULK2 in human brain tissue with ULK2 antibody at 2.5 μg/ml.
WB
(A, B) ULK2 and GABARAPL1 expression decreased in OC tissues, compared to the paired paracancerous tissues specimens from 8 patients, determined by qRT-PCR analysis and western blot analysis.
IHC
(C) Representative immunohistochemical images of ULK2 and GABARAPL1 expression in OC tissues and paracancerous tissues analyzed by IHC (400×).| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | ULK2 Antibody can be used for detection of ULK2 by Western blot at 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ULK2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | ULK2 Antibody is supplied in PBS containing 0.02% sodium azide. |
