Application
Western blot analysis of ULK3 in human brain tissue lysate with ULK3 antibody at (A) 0.5 and (B) 1 μg/ml.Application
Immunofluorescence of ULK3 in human brain tissue with ULK3 antibody at 20 μg/mL.Application
Immunohistochemistry of ULK3 in human brain tissue with ULK3 antibody at 5 μg/mL.| Product Name | ULK3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ULK3 Antibody: Unc-51-like kinase 3 |
| Product description | ULK3 belongs to the Ser/Thr protein kinase superfamily and plays a role in the ATP-dependent phosphorylation of target proteins (1). Knockout of ULK genes results in a severe defect in the autophagy pathway (2). ULK3, like the other Unc-51-like kinases such as ULK1, ULK2 and ULK4, is highly conserved among eukaryotes (3). ULK3 has been shown to be a positive regulator of the Hedgehog signaling pathway by enhancing GLI1 and GLI2 transcriptional activity (4). Furthermore, ULK3 can also interact with SUFU, a protein required for the negative regulation of GLI proteins; this interaction blocks the autophosphorylation of ULK3 and blocks its ability to regulate the GLI proteins (5).1) Suzuki K, Kubota Y, Sekito T, et al. Hierarchy of Atg proteins in pre-autophagosomal structure organization. Genes to Cells 2007; 12:209–18. |
| Immunogen | ULK3 antibody was raised against a 19 amino acid peptide near the center of human ULK3. The immunogen is located within amino acids 230 - 280 of ULK3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ULK3 antibody can be used for detection of ULK3 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ULK3 |
|---|---|
| Alternative Names | ULK3 Antibody: Unc-51-like kinase 3 |
| Molecular Weight(MW) | Predicted: 52 kDa Observed: 52 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | ULK3 antibody is human specific. Multiple isoforms of ULK3 are known to exist. |
| Entrez Gene | 25989 |
|---|---|
| Protein Accession | NP_001092906 |
Application
Western blot analysis of ULK3 in human brain tissue lysate with ULK3 antibody at (A) 0.5 and (B) 1 μg/ml.
Application
Immunofluorescence of ULK3 in human brain tissue with ULK3 antibody at 20 μg/mL.
Application
Immunohistochemistry of ULK3 in human brain tissue with ULK3 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | ULK3 antibody can be used for detection of ULK3 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ULK3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | ULK3 antibody is supplied in PBS containing 0.02% sodium azide. |
