Application
Western blot analysis of UNC5C Antibody in K562 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human brain tissue with UNC5C Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of K562 cells using UNC5C Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | UNC5C Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Netrin receptor UNC5C, Protein unc-5 homolog 3, Protein unc-5 homolog C, UNC5C, UNC5H3 |
| Product description | UNC5C belongs to the UNC-5 family of netrin receptors. Netrins are secreted proteins that direct axon extension and cell migration during neural development. They are bifunctional proteins that act as attractants for some cell types and as repellents for others, and these opposite actions are thought to be mediated by two classes of receptors. The UNC-5 family of receptors mediate the repellent response to netrin; they are transmembrane proteins containing 2 immunoglobulin (Ig)-like domains and 2 type I thrombospondin motifs in the extracellular region.1) Hibi,K., et.al., WorldSurg 33 (5), 1053-1057 (2009) |
| Immunogen | This UNC5C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-217 amino acids from the Central region of human UNC5C. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UNC5C |
|---|---|
| Alternative Names | Netrin receptor UNC5C Protein unc-5 homolog 3 Protein unc-5 homolog C UNC5C UNC5H3 |
| Molecular Weight(MW) | 103 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Western blot analysis of UNC5C Antibody in K562 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human brain tissue with UNC5C Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of K562 cells using UNC5C Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
