Application
Western blot analysis of UNG2 in 3T3 cell lysate with UNG2 antibody at 1 μg/mL in (A) the presence and (B) the absence of blocking peptide.Application
Immunofluorescence of UNG2 in 3T3 cells with UNG2 antibody at 20 μg/mL.| Product Name | UNG2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UNG2 Antibody: DGU, UDG, UNG1, UNG2, HIGM4, HIGM5, UNG15, DGU, Uracil-DNA glycosylase |
| Product description | UNG2 Antibody: The human uracil-DNA glycosylase (UNG) gene encodes both mitochondrial (UNG1) and nuclear (UNG2) forms through differentially regulated promoters and alternative splicing. UNG2 is the major enzyme in the base excision repair pathway that removes uracil residues from DNA that arise through either misincorporation during replication or cytosine deamination. UNG2 can also be bound by the HIV-1 integrase and incorporated into the virion particle, suggesting that it is required to remove uracils from the viral genome. As the intrinsic antiviral protein APOBEC3G generates numerous uracils in the HIV genome during its replication, it may be that the UNG2 contributes to the APOBEC3G-mediated loss of infectivity by generating abasic sites in the viral genome.1) Krokan HE, Otterlei M, Nilsen H, et al. Properties and functions of human uracil-DNA glycosylase from the UNG gene. Prog. Nucleic Acid Res. Mol. Biol. 2001; 68:365-86. |
| Immunogen | UNG2 antibody was raised against a 17 amino acid peptide near the amino terminus of human UNG2. The immunogen is located within the first 50 amino acids of UNG2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| UNG2 antibody can be used for detection of UNG2 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UNG |
|---|---|
| Alternative Names | UNG2 Antibody: DGU UDG UNG1 UNG2 HIGM4 HIGM5 UNG15 DGU Uracil-DNA glycosylase |
| Molecular Weight(MW) | Predicted: 34 kDa Observed: 33 kDa |
| Sequence Similarities | 2) Cat. No. 17-201 - 3T3/BALB Cell Slide |
| Tissue Specificity | At least two isoforms of UNG2 are known to exist; this antibody will only detect the longer isoform. UNG2 antibody is predicted to not cross-react with UNG1. |
Application
Western blot analysis of UNG2 in 3T3 cell lysate with UNG2 antibody at 1 μg/mL in (A) the presence and (B) the absence of blocking peptide.
Application
Immunofluorescence of UNG2 in 3T3 cells with UNG2 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1212 - 3T3 Cell Lysate |
|---|---|
| Application Notes | UNG2 antibody can be used for detection of UNG2 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | UNG2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | UNG2 Antibody is supplied in PBS containing 0.02% sodium azide. |
