Application
Western blot analysis of lysate from R. heart cell line, using USP2 Antibody (T22) at 1:1000.Application
Western blot analysis of USP2 Antibody in HL60 cell line lysates (35ug/lane)Application
IHC for USP2a in a formalin-fixed, paraffin-embedded human prostate sample. Cytoplasmic positive immunostaining was detected in tumor glands (ii), whereas in normal prostatic epithelium, USP2a expression was restricted to the basal layer.Application
Western blot analysis of USP2 using rabbit polyclonal USP2 Antibody (T22) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the USP2 gene.| Product Name | USP2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ubiquitin carboxyl-terminal hydrolase 2, 41 kDa ubiquitin-specific protease, Deubiquitinating enzyme 2, Ubiquitin thioesterase 2, Ubiquitin-specific-processing protease 2, USP2, UBP41 |
| Product description | Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.1) Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). |
| Immunogen | This USP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-101 amino acids from the N-terminal region of human USP2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Gene Symbol | USP2 |
|---|---|
| Alternative Names | Ubiquitin carboxyl-terminal hydrolase 2 41 kDa ubiquitin-specific protease Deubiquitinating enzyme 2 Ubiquitin thioesterase 2 Ubiquitin-specific-processing protease 2 USP2 UBP41 |
| Molecular Weight(MW) | 68 kDa |
Application
Western blot analysis of lysate from R. heart cell line, using USP2 Antibody (T22) at 1:1000.
Application
Western blot analysis of USP2 Antibody in HL60 cell line lysates (35ug/lane)
Application
IHC for USP2a in a formalin-fixed, paraffin-embedded human prostate sample. Cytoplasmic positive immunostaining was detected in tumor glands (ii), whereas in normal prostatic epithelium, USP2a expression was restricted to the basal layer.
Application
Western blot analysis of USP2 using rabbit polyclonal USP2 Antibody (T22) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the USP2 gene.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
