Application
Western blot analysis of lysed extracts from C6 cells untreated or treated with PMA using VASP (Phospho-Ser157).Application
Immunohistochemical analysis of paraffin-embedded human tonsil carcinoma tissue using VASP (Phospho-Ser157).| Product Name | VASP (phospho Ser157) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | STAT5, |
| Product description | Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.1) Zhao WM, et al. (2001) EMBO J 20 (9): 2315-2325. Millard TH, et al. (2005) EMBO J 24 (2): 240-250. Kühnel K, et al. (2004) Proc Natl Acad Sci U S A 101 (49): 17027-17032. Haffner C, et al. (1995) EMBO J 14 (1): 19-27. Ramesh N, et al. (1997) Proc Natl Acad Sci U S A 94 (26): 14671-14676. |
| Immunogen | VASP (Phospho-Ser157) antibody was raised against a peptide sequence around phosphorylation site of serine 157 (R-V-S (p) -N-A) derived from Human VASP. |
| Modification | p-Ser157 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VASP |
|---|---|
| Alternative Names | STAT5 |
| Molecular Weight(MW) | 50 kDa |
| Tissue Specificity | This antibody detects endogenous level of VASP only when phosphorylated at serine 157. |
| Entrez Gene | 7408 |
|---|---|
| Protein Accession | NP_003361.1 |
Application
Western blot analysis of lysed extracts from C6 cells untreated or treated with PMA using VASP (Phospho-Ser157).
Application
Immunohistochemical analysis of paraffin-embedded human tonsil carcinoma tissue using VASP (Phospho-Ser157).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
