Application
Western blot analysis of lysed extracts from serum-treated 293 and PMA-treated C6 cells using VASP (Phospho-Ser239).Application
Immunohistochemical analysis of paraffin-embedded human tonsil carcinoma tissue using VASP (Phospho-Ser239).| Product Name | VASP (phospho Ser239) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NFKB3, |
| Product description | Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.1) Wang HG, et al. (2005) euron; 45 (3): 389-403. Melichar VO, et al. (2004) Proc Natl Acad Sci U S A; 101 (47): 16671-6. Jensen BO, et al. (2004) Blood; 104 (9): 2775-82. Zhuang S, et al. (2004) J Biol Chem; 279 (11): 10397-407. Smolenski A, et al. (1998) J Biol Chem; 273 (32): 20029-35. Butt E, et al. (1994) J Biol Chem; 269 (20): 14509-17. |
| Immunogen | VASP (Phospho-Ser239) antibody was raised against a peptide sequence around phosphorylation site of serine 238 (K-V-S (p) -K-Q) derived from Human VASP. |
| Modification | p-Ser239 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VASP |
|---|---|
| Alternative Names | NFKB3 |
| Molecular Weight(MW) | 50 kDa |
| Tissue Specificity | This antibody detects endogenous level of VASP only when phosphorylated at serine 239. |
| Entrez Gene | 7408 |
|---|---|
| Protein Accession | NP_003361.1 |
Application
Western blot analysis of lysed extracts from serum-treated 293 and PMA-treated C6 cells using VASP (Phospho-Ser239).
Application
Immunohistochemical analysis of paraffin-embedded human tonsil carcinoma tissue using VASP (Phospho-Ser239).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
