Application
Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: HUVEC whole cell lysate Lane 5: MDA-MB-453 whole cell lysate Lane 6: THP-1 whole cell lysate Lysates/proteins at 20 ug per lane.Application
VASP Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | VASP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Vasodilator-stimulated phosphoprotein, VASP, VASP |
| Product description | Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.1) Barragan, P., et al. Thromb. Haemost. 104(2):410-411(2010) |
| Immunogen | This VASP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 267-296 amino acids from the C-terminal region of human VASP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VASP |
|---|---|
| Alternative Names | Vasodilator-stimulated phosphoprotein VASP VASP |
| Molecular Weight(MW) | 40 kDa |
Application
Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: HUVEC whole cell lysate Lane 5: MDA-MB-453 whole cell lysate Lane 6: THP-1 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
VASP Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
