Application
Western blot analysis of lysed extracts from Jurkat cells untreated or treated with H2O2 using Vav (Phospho-Tyr174).| Product Name | Vav (phospho Tyr174) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | VAV, P53, VAV |
| Product description | Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation1) Amarasinghe GK, et al. (2005) Biochemistry; 44 (46): 15257-68. Charvet C, et al. (2005) J Biol Chem; 280 (15): 15289-99. Miura-Shimura Y, et al. (2003) J Biol Chem; 278 (40): 38495-504. Kuhne MR, et al. (2000) J Biol Chem; 275 (3): 2185-90. |
| Immunogen | Vav (Phospho-Tyr174) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 174 (E-I-Y (p) -E-D) derived from Human Vav. |
| Modification | p-Tyr174 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VAV1 |
|---|---|
| Alternative Names | VAV P53 VAV |
| Molecular Weight(MW) | 95 kDa |
| Tissue Specificity | This antibody detects endogenous level of Vav only when phosphorylated at tyrosine 174. |
| Entrez Gene | 7409 |
|---|---|
| Protein Accession | NP_005419.2 |
Application
Western blot analysis of lysed extracts from Jurkat cells untreated or treated with H2O2 using Vav (Phospho-Tyr174).| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
