Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with VEGF2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | VEGFB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Vascular endothelial growth factor B, VEGF-B, VEGF-related factor, VRF, VEGFB, VRF |
| Product description | Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGFs stimulate endothelial cells, induce angiogenesis, promote cell migration, increase vascular permeability, and inhibit apoptosis. VEGFB has structural similarities to VEGF and PIGF and is frequently co-expressed with VEGF. There are two alternatively spliced isoforms of VEGFB: VEGFB 167 and VEGFB 186. VEGFB 167, a highly basic heparin-binding protein, remains with the cell or extracellular matrix whereas, VEGFB 186 is readily secreted. VEGFB stimulates endothelial cell proliferation. VEGFB binds to the tyrosine kinase receptor VEGFR1 (flt1) and does not bind to VEGFR2. Vascular Endothelial Growth Factor B is widely expressed but is most abundant in heart, skeletal muscle, and pancreas. It has been suggested that VEGFB expression in human primary breast cancers is associated with lymph node metastasis. The genes that encode VEGFB have been mapped to chromosome 11q13.1) Trompezinski, S., et al., Exp. Dermatol. 13(2):98-105 (2004). |
| Immunogen | This VEGFB (VEGF2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110-139 amino acids from the Central region of human VEGFB (VEGF2). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | VEGFB |
|---|---|
| Alternative Names | Vascular endothelial growth factor B VEGF-B VEGF-related factor VRF VEGFB VRF |
| Molecular Weight(MW) | 22 kDa |
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with VEGF2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
