Application
Western blot analysis of lysed extracts from SKOV3 cells using VEGFR2 (Ab-951).Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using VEGFR2 (Ab-951). Bottom Image: Immunofluorescence staining of methanol-fixed MCF cells using VEGFR2 (Ab-951).| Product Name | VEGFR2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLK1, CD309, VEGFR, VEGFR2, CK2A1, FLK1, KDR, VGFR2, VGR2, kinase insert domain receptor |
| Product description | Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions1) Zeng H, et al. (2001) J Biol Chem. 276 (35): 32714-32719. Dougher M, et al. (1999) Oncogene. 18 (8): 1619-1627. |
| Immunogen | VEGFR2 (Ab-951) antibody was raised against a peptide sequence around aa.949~953 (K-D-Y-V-G) derived from Human VEGFR2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KDR |
|---|---|
| Alternative Names | FLK1 CD309 VEGFR VEGFR2 CK2A1 FLK1 KDR VGFR2 VGR2 kinase insert domain receptor |
| Molecular Weight(MW) | 230 kDa |
| Tissue Specificity | This antibody detects endogenous level of total VEGFR2 protein. |
| Entrez Gene | 3791 |
|---|---|
| Protein Accession | NP_002244.1 |
Application
Western blot analysis of lysed extracts from SKOV3 cells using VEGFR2 (Ab-951).
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using VEGFR2 (Ab-951). Bottom Image: Immunofluorescence staining of methanol-fixed MCF cells using VEGFR2 (Ab-951).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
