Application
Western blot analysis of VGF in human brain tissue lysate with VGF antibody at (A) 0.5 and (B) 1 μg/mL.Application
Immunofluorescence of VGF in Human Brain cells with VGF antibody at 20 μg/mL.Application
Immunohistochemistry of VGF in human brain with VGFantibody at 5 μg/mL.| Product Name | VGF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | VGF Antibody: Neurosecretory protein VGF, NERP-1 |
| Product description | VGF Antibody: VGF was initially identified as a rapidly regulated gene product in nerve growth factor-treated PC12 cells. This protein is synthesized in neurons in the central and peripheral nervous system as well as in the pituitary, adrenal medulla, endocrine cells of the stomach, and pancreatic beta cells. VGF is thought to be involved in organism energy balance and regulation of homeostasis as mice lacking this gene show deficits in these areas. More recent results suggest that VGF is upregulated by brain-derived neurotrophic factor (BDNF) and can stimulate the proliferation of hippocampal progenitor cells and produce antidepressant-like behavioral effects, suggesting that this pathway may be a suitable target for therapeutic treatments.1) Levi A, Eldridge JD, and Paterson BM. Molecular cloning of a gene sequence regulated by nerve growth factor. Science 1985; 229:393-5. |
| Immunogen | VGF antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of the human VGF. The immunogen is located within the last 50 amino acids of VGF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| VGF antibody can be used for detection of VGF by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VGF |
|---|---|
| Alternative Names | VGF Antibody: Neurosecretory protein VGF NERP-1 |
| Molecular Weight(MW) | Predicted: 68 kDa Observed: 80 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Western blot analysis of VGF in human brain tissue lysate with VGF antibody at (A) 0.5 and (B) 1 μg/mL.
Application
Immunofluorescence of VGF in Human Brain cells with VGF antibody at 20 μg/mL.
Application
Immunohistochemistry of VGF in human brain with VGFantibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | VGF antibody can be used for detection of VGF by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | VGF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | VGF Antibody is supplied in PBS containing 0.02% sodium azide. |
