Application
Western blot analysis of extracts from A549 cells treated with Nocodazole using Phospho-Vimentin (Ser56) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | Vimentin (phospho Ser56) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | VIME, vimentin |
| Product description | Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.1) Matsuyama M, et al. (2013)J Biol Chem 288, 35626-35. Haolong C, et al. (2013)PLoS One 8, e73900. Kotula E, et al. (2013)PLoS One 8, e80313. |
| Immunogen | Vimentin (phospho Ser56) antibody was raised against a peptide sequence around phosphorylation site of serine 56 (A-S-S (p)-P-G) derived from Human Vimentin. |
| Modification | p-Ser56 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VIM |
|---|---|
| Alternative Names | VIME vimentin |
| Molecular Weight(MW) | 54 kDa |
| Tissue Specificity | The antibody detects endogenous level of Vimentin only when phosphorylated at serine 56. |
| Entrez Gene | 7431 |
|---|---|
| Protein Accession | NP_003371.2 |
Application
Western blot analysis of extracts from A549 cells treated with Nocodazole using Phospho-Vimentin (Ser56) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
