Application
Western blot analysis of extract from HeLa, cos7 and JK cells using Vimentin Antibody.| Product Name | Vimentin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HEL113, CTRCT30, FLJ36605, VIM |
| Product description | Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.1) Sommers C. L., Walker-Jones D., Heckford S. E., Worland P., Valverius E., Clark R., McCormick F., Cancer Res. 49:4258-4263 (1989) Kang S. -M., Shin M. -J., Kim J. -H., Oh J. -W. Proteomics 5:2227-2237 (2005) Ahmed B. A., Bukhari I. A., Jeffus B. C., Harney J. T., Thyparambil S., Ziu E., Fraer M., Rusch N. J., PLoS ONE 4:E4730-E4730 (2009) |
| Immunogen | Vimentin antibody was raised against a peptide sequence around aa.452~456 (G-Q-V-I-N) derived from Human Vimentin. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VIM |
|---|---|
| Alternative Names | HEL113 CTRCT30 FLJ36605 VIM |
| Molecular Weight(MW) | 57 kDa |
| Tissue Specificity | This antibody detects endogenous level of total Vimentin protein. |
| Entrez Gene | 7431 |
|---|---|
| Protein Accession | NP_003371.2 |
Application
Western blot analysis of extract from HeLa, cos7 and JK cells using Vimentin Antibody.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
