Application
Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysates Lane 2: 293T/17 whole cell lysates Lane 3: MCF-7 whole cell lysates Lysates/proteins at 20 ug per lane.| Product Name | VRK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Serine/threonine-protein kinase VRK1, Vaccinia-related kinase 1, VRK1 |
| Product description | This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN.1) Nezu, J., et al., Genomics 45(2):327-331 (1997). |
| Immunogen | This VRK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-396 amino acids from the Central region of human VRK1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VRK1 |
|---|---|
| Alternative Names | Serine/threonine-protein kinase VRK1 Vaccinia-related kinase 1 VRK1 |
| Molecular Weight(MW) | 45 kDa |
Application
Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysates Lane 2: 293T/17 whole cell lysates Lane 3: MCF-7 whole cell lysates Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
