Application
Western blot analysis of WAS Antibody in mouse liver tissue lysates (35ug/lane)Application
WAS Antibody IHC analysis in formalin fixed and paraffin embedded tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | WAS Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Wiskott-Aldrich syndrome protein, WASp, WAS, IMD2 |
| Product description | The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients.1) Rajmohan, R., et al. FEMS Yeast Res. 9(8):1226-1235(2009) |
| Immunogen | This WAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-144 amino acids from the Central region of human WAS. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WAS |
|---|---|
| Alternative Names | Wiskott-Aldrich syndrome protein WASp WAS IMD2 |
| Molecular Weight(MW) | 53 kDa |
Application
Western blot analysis of WAS Antibody in mouse liver tissue lysates (35ug/lane)
Application
WAS Antibody IHC analysis in formalin fixed and paraffin embedded tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
