Application
Western blot analysis of WDR5 in 293 cell lysate with WDR5 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of WDR5 in 293 cells with WDR5 antibody at 20 μg/mL.Application
Immunocytochemistry of WDR5 in 293 cells with WDR5 antibody at 5 μg/mL.| Product Name | WDR5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WDR5 Antibody: SWD3, BIG-3, BIG3, WD repeat-containing protein 5, BMP2-induced 3-kb gene protein |
| Product description | WDR5 Antibody: WD repeat domain 5 (WDR5) is a member of the WD repeat protein family, which is involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. WDR5, also known as BIG-3, is expressed in the developing growth plate, accelerates chondrocyte and osteoblast differentiation in vitro, and regulates osteoblast differentiation during embryonic bone development. WDR5 interacts with the pluripotency factor Oct4/POU5F1 and is required for the efficient formation of induced pluripotent stem (iPS) cells.1) Smith TF, Gaitatzes C, Saxena K, et al. The WD repeat: a common architecture for diverse functions. Trends Biochem. Sci. 1999; 24:181-5. |
| Immunogen | WDR5 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human WDR5. The immunogen is located within the first 50 amino acids of WDR5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| WDR5 antibody can be used for detection of WDR5 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WDR5 |
|---|---|
| Alternative Names | WDR5 Antibody: SWD3 BIG-3 BIG3 WD repeat-containing protein 5 BMP2-induced 3-kb gene protein |
| Sequence Similarities | 2) Cat. No. 17-010 - HEK293 Cell Slide |
| Tissue Specificity | WDR5 antibody is human specific. WDR5 antibody is predicted to not cross-react with other WDR family members. |
Application
Western blot analysis of WDR5 in 293 cell lysate with WDR5 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of WDR5 in 293 cells with WDR5 antibody at 20 μg/mL.
Application
Immunocytochemistry of WDR5 in 293 cells with WDR5 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1210 - HEK293 Cell Lysate |
|---|---|
| Application Notes | WDR5 antibody can be used for detection of WDR5 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | WDR5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | WDR5 Antibody is supplied in PBS containing 0.02% sodium azide. |
