Application
Western blot analysis of WNV Core in (A) untransfected or (B) transfected HeLa lysate with WNV Core antibody at 1 μg/mL.Application
Immunocytochemical staining of transfected Vero cells using WNV Core antibody at 20 μg/mL.| Product Name | West Nile Virus Core Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | West Nile Virus Core Antibody: Genome polyprotein, Core protein, NS1 |
| Product description | West Nile Virus Core Antibody: West Nile Virus (WNV) is a member of the Flaviviridae, a plus-stranded virus family that includes St. Louis encephalitis virus, yellow fever virus, and Dengue virus. WNV was initially isolated in 1937 in the West Nile region of Uganda and has become prevalent in Africa, Asia, and Europe. It has rapidly spread across the United States with cases being observed in every continental state. Virus particles consist of a dense core made up of the core/capsid protein encapsulating the RNA genome surrounded by a membrane envelope embedded with envelope and matrix proteins which play a major role for WNV entry into target cells. The viral core protein is thought to contribute to the WNV-associated inflammation via apoptosis induced though the caspase-9 pathway as delivery of core gene delivery into the striatum of mouse brain and skeletal muscle resulted in cell death and inflammation.1) Gould LH and Fikrig E. West Nile virus: a growing concern. J. Clin. Invest. 2004; 113:1102-7. |
| Immunogen | West Nile Virus Core antibody was raised against a synthetic peptide corresponding to 15 amino acids near the carboxy terminus of the West Nile Virus core protein. The immunogen is located within amino acids 80 - 130 of West Nile Virus Core. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| West Nile virus Core antibody can be used for the detection of the WNV Core protein in ELISA. It will detect 10 ng of free peptide at 1 μg/mL. This antibody will also detect the protein in immunoblot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL.: |
|
| Species Reactivity | Virus |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WNVgp1 |
|---|---|
| Alternative Names | West Nile Virus Core Antibody: Genome polyprotein Core protein NS1 |
Application
Western blot analysis of WNV Core in (A) untransfected or (B) transfected HeLa lysate with WNV Core antibody at 1 μg/mL.
Application
Immunocytochemical staining of transfected Vero cells using WNV Core antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 17-008 - THP-1 Cell Slide |
|---|---|
| Application Notes | West Nile virus Core antibody can be used for the detection of the WNV Core protein in ELISA. It will detect 10 ng of free peptide at 1 μg/mL. This antibody will also detect the protein in immunoblot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | West Nile Virus Core antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | West Nile Virus Core Antibody is supplied in PBS containing 0.02% sodium azide. |
