Application
Western blot analysis of WFDC2 in A549 cell lysate with WFDC2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of WFDC2 in human lung tissue with WFDC2 antibody at 20 μg/mL.Application
Immunohistochemistry of WFDC2 in human lung tissue with WFDC2 antibody at 2.5 μg/mL.| Product Name | WFDC2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WFDC2 Antibody : HE4, WAP5, EDDM4, dJ461P17.6, HE4, WAP four-disulfide core domain protein 2, Epididymal secretory protein E4 |
| Product description | The WAP four-disulfide core domain protein 2 (WFDC2), also known as epididymal protein 4, is a member of the WFDC domain family, a family of proteins that is characterized by the presence of Whey Acidic Protein (WAP) domain, and is highly expressed in the lung and salivary gland (1,2). Members of this family include SLPI and elafin, antiproteinases involved in the innate immune system (2). WFDC2 has been proposed to play a critical role in tumor formation and growth in ovarian cancer cells through the regulation of growth- and apoptosis-associated genes and may thus be a potential therapeutic target for epithelial ovarian cancer (3).1) Drapkin R, von Horsten HH, Lin Y, et al. Human epididymis protein 4 (HE4) is a secreted glycoprotein that is overexpressed by serious and endometrioid ovarian carcinomas. Cancer Res. 2005; 65:2162-9. |
| Immunogen | WFDC2 antibody was raised against a 16 amino acid peptide near the amino terminus of human WFDC2. The immunogen is located within the first 50 amino acids of WFDC2 . |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| WFDC2 antibody can be used for detection of WFDC2 by Western blot at 1 - 2 μg/ml.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WFDC2 |
|---|---|
| Alternative Names | WFDC2 Antibody : HE4 WAP5 EDDM4 dJ461P17.6 HE4 WAP four-disulfide core domain protein 2 Epididymal secretory protein E4 |
| Molecular Weight(MW) | Predicted: 14 kDa Observed: 20kDa |
| Sequence Similarities | 2) Cat. No. 10-101 - Human Lung Tissue Slide |
| Tissue Specificity | WFDC2 antibody is human specific. Multiple isoforms of WFDC2 are known to exist. |
Application
Western blot analysis of WFDC2 in A549 cell lysate with WFDC2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of WFDC2 in human lung tissue with WFDC2 antibody at 20 μg/mL.
Application
Immunohistochemistry of WFDC2 in human lung tissue with WFDC2 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1203 - A549 Cell Lysate |
|---|---|
| Application Notes | WFDC2 antibody can be used for detection of WFDC2 by Western blot at 1 - 2 μg/ml.: |
| Form | Liquid |
|---|---|
| Storage Instructions | WFDC2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | WFDC2 Antibody is supplied in PBS containing 0.02% sodium azide. |
