Application
Western blot analysis of WNT1 Antibody in mouse heart tissue lysates (35ug/lane)Application
WNT1 Antibody IHC analysis in formalin fixed and paraffin embedded human testis followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | WNT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Proto-oncogene Wnt-1, Proto-oncogene Int-1 homolog, WNT1, INT1 |
| Product description | WNT1 is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum.1) Shuai,X., et.al., Cancer Genet. Cytogenet. 194 (2), 119-124 (2009) |
| Immunogen | This WNT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260-288 amino acids from the C-terminal region of human WNT1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | WNT1 |
|---|---|
| Alternative Names | Proto-oncogene Wnt-1 Proto-oncogene Int-1 homolog WNT1 INT1 |
| Molecular Weight(MW) | 41 kDa |
Application
Western blot analysis of WNT1 Antibody in mouse heart tissue lysates (35ug/lane)
Application
WNT1 Antibody IHC analysis in formalin fixed and paraffin embedded human testis followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
