Application
Western blot analysis of Wnt10a in RAW264.7 cell lysate with Wnt10a antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of Wnt10a in Human Skeletal Muscle tissue with Wnt10a antibody at 20 μg/mL.Application
Immunohistochemistry of Wnt10a in mouse skeletal muscle tissue with Wnt10a antibody at 5 μg/ml.Application
Immunohistochemistry of Wnt10a in human skeletal muscle tissue with Wnt10a antibody at 10 μg/mL.| Product Name | Wnt10a Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Wnt10a Antibody: OODD, SSPS, STHAG4, Protein Wnt-10a |
| Product description | Wnt10a Antibody: Wnt10a is a member of the Wnt family, a gene family that encodes secreted signaling proteins that play crucial roles in normal development such as regulation of cell fate and patterning during embryogenesis as well as neoplastic transformation. Elevated levels of Wnt10a have been detected in several human tumors and cancer cell lines including colorectal cancer and chronic lymphocytic leukemia and it has been suggested that uncontrolled Wnt signaling may contribute to the defect in apoptosis that characterizes these malignancies. Recently, a mutation in Wnt10a was found to be associated with odonto-onycho-dermal dysplasia, an autosomal recessive ectodermal dysplasia.1) Kelly GM, Lai CJ, and Moon RT. Expression of wnt10a in the central nervous system of developing zebrafish. Dev. Biol. 1993; 158:113-21. |
| Immunogen | Wnt10a antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Wnt10a. The immunogen is located within amino acids 300 - 350 of Wnt10a. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Wnt10a antibody can be used for detection of Wnt10a by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WNT10A |
|---|---|
| Alternative Names | Wnt10a Antibody: OODD SSPS STHAG4 Protein Wnt-10a |
| Molecular Weight(MW) | Predicted: 46 kDa Observed: 46 kDa |
| Tissue Specificity | This Wnt10a antibody is predicted to not cross-react with Wnt10b. |
Application
Western blot analysis of Wnt10a in RAW264.7 cell lysate with Wnt10a antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of Wnt10a in Human Skeletal Muscle tissue with Wnt10a antibody at 20 μg/mL.
Application
Immunohistochemistry of Wnt10a in mouse skeletal muscle tissue with Wnt10a antibody at 5 μg/ml.
Application
Immunohistochemistry of Wnt10a in human skeletal muscle tissue with Wnt10a antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1283 - RAW 264.7 Cell Lysate |
|---|---|
| Application Notes | Wnt10a antibody can be used for detection of Wnt10a by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Wnt10a antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Wnt10a Antibody is supplied in PBS containing 0.02% sodium azide. |
