Application
Western blot analysis of WNT4 antibody in 293 cell line lysates (35ug/lane)Application
(LEFT)Formalin-fixed and paraffin-embedded human lung carcinoma reacted with WNT4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (RIGHT)Flow cytometric analysis of 293 cells using WNT4 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | WNT4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein Wnt-4, WNT4 |
| Product description | The WNT family consists of secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT4 is a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. It play a concerted role in both the control of female development and the prevention of testes formation.1) Kuulasmaa,T., Horm. Metab. Res. 40 (10), 668-673 (2008) Miyakoshi,T., Endocr. Pathol. 19 (4), 261-273 (2008) |
| Immunogen | This WNT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242-269 amino acids from the C-terminal region of human WNT4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | WNT4 |
|---|---|
| Alternative Names | Protein Wnt-4 WNT4 |
| Molecular Weight(MW) | 39 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis of WNT4 antibody in 293 cell line lysates (35ug/lane)
Application
(LEFT)Formalin-fixed and paraffin-embedded human lung carcinoma reacted with WNT4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (RIGHT)Flow cytometric analysis of 293 cells using WNT4 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
