Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Product Name | WNT5B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WNT5B, |
| Product description | The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT5B gene is a member of the WNT gene family. WNT5B shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants.1) Mangioni, S., (2005) J. Clin. Endocrinol. Metab. 90 (9), 5349-5355. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human WNT5B. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WNT5B antibody can be used for detection of WNT5B by ELISA at 1:312500. WNT5B antibody can be used for detection of WNT5B by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | WNT5B |
|---|---|
| Alternative Names | WNT5B |
| Molecular Weight(MW) | 39 kDa, 40 kDa |
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | WNT5B antibody can be used for detection of WNT5B by ELISA at 1:312500. WNT5B antibody can be used for detection of WNT5B by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store WNT5B antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
