WNT9B is a ligand for members of the frizzled family of seven transmembrane receptors. WNT9B is a probable developmental protein. WNT9B may be a signaling molecule which affects the development of discrete regions of tissues. WNT9B is likely to signal over only few cell diameters.The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Study of its expression in the teratocarcinoma cell line NT2 suggests that it may be implicated in the early process of neuronal differentiation of NT2 cells induced by retinoic acid. This gene is clustered with WNT3, another family member, in the chromosome 17q21 region.1) Chiquet, B.T., (er) Hum. Mol. Genet. (2008) In press.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human WNT9B.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
WNT9B antibody can be used for detection of WNT9B by ELISA at 1:312500. WNT9B antibody can be used for detection of WNT9B by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.
Positive Control
1) Tranfected 293T Cell Lysate
Application Notes
WNT9B antibody can be used for detection of WNT9B by ELISA at 1:312500. WNT9B antibody can be used for detection of WNT9B by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store WNT9B antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.