Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.| Product Name | WT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | WT1 is a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms.This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.1) Ruteshouser, E.C., (2008) Genes Chromosomes Cancer 47 (6), 461-470. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human WT1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WT1 antibody can be used for detection of WT1 by ELISA at 1:312500. WT1 antibody can be used for detection of WT1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WT1 |
|---|---|
| Alternative Names | WT1 GUD WAGR WIT-2 WT33 AWT1 NPHS4 EWS-WT1 |
| Molecular Weight(MW) | 56 kDa |
Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | WT1 antibody can be used for detection of WT1 by ELISA at 1:312500. WT1 antibody can be used for detection of WT1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store WT1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
