Application
Western blot analysis of WWC1 in human brain tissue lysate with WWC1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of WWC1 in mouse brain tissue with WWC1 antibody at 20 μg/ml.Application
Immunohistochemistry of WWC1 in mouse brain tissue with WWC1 antibody at 5 μg/ml.| Product Name | WWC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WW and C2 domain containing 1, HBEBP3, HBEBP36, KIBRA, MEMRYQTL |
| Product description | The WW and C2 domain containing 1 (WWC1) protein, also known as KIBRA, possesses two WW domains and an internal C2-like domain (1). WWC1 was originally identified as a memory performance-associated protein in humans (2) and has recently been shown to be a novel regulator of the Hippo pathway (3). WWC1 is phosphorylated by the mitotic kinases Aurora-A and –B (4), and in turn activates the Aurora kinases and is required for precise chromosome alignment during mitosis (5).1) Kremerskothen J, Plaas C, Buther K, et al. Characterization of KIBRA, a novel WW domain-containing protein. Biochem. Biophys. Res. Commun. 2003; 300:862-7. |
| Immunogen | WWC1 antibody was raised against an 18 amino acid peptide near the amino terminus of human WWC1. The immunogen is located within amino acids 60 - 110 of WWC1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| WWC1 antibody can be used for detection of WWC1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WWC1 |
|---|---|
| Alternative Names | WW and C2 domain containing 1 HBEBP3 HBEBP36 KIBRA MEMRYQTL |
| Molecular Weight(MW) | Predicted: 123 kDa Observed: 110 kDa |
| Tissue Specificity | WWC1 antibody is human, mouse and rat reactive. At least three isoforms of WWC1 are known to exist; this antibody will detect all three isoforms. |
| Entrez Gene | 23286 |
|---|---|
| Protein Accession | NP_001155133 |
Application
Western blot analysis of WWC1 in human brain tissue lysate with WWC1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of WWC1 in mouse brain tissue with WWC1 antibody at 20 μg/ml.
Application
Immunohistochemistry of WWC1 in mouse brain tissue with WWC1 antibody at 5 μg/ml.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | WWC1 antibody can be used for detection of WWC1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | WWC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | WWC1 antibody is supplied in PBS containing 0.02% sodium azide. |
