Application
Western blot analysis of XBP-1 in HepG2 cell lysate with XBP-1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.Application
Immunofluorescence of XBP-1 in HepG2 cells with XBP-1 antibody at 20 μg/ml.Application
Immunocytochemistry of XBP-1 in HepG2 cells with XBP-1 antibody at 10 μg/mL.| Product Name | XBP-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | XBP-1 Antibody: XBP2, TREB5, XBP-1, XBP2, X-box-binding protein 1, Tax-responsive element-binding protein 5 |
| Product description | XBP-1 Antibody: X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER). Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.1) Yoshida H, Matsui T, Yamamoto T, et al. XBP1 mRNA is induced by ATF6 and spliced by IRE1p in response to ER stress to produce a highly active transcription factor. Cell 2001; 107:881-91. |
| Immunogen | XBP-1 antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human XBP-1. The immunogen is located within amino acids 40 - 90 of XBP-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| XBP-1 antibody can be used for the detection of XBP-1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | XBP1 |
|---|---|
| Alternative Names | XBP-1 Antibody: XBP2 TREB5 XBP-1 XBP2 X-box-binding protein 1 Tax-responsive element-binding protein 5 |
| Sequence Similarities | 2) Cat. No. 17-011 - Hep G2 Cell Slide |
Application
Western blot analysis of XBP-1 in HepG2 cell lysate with XBP-1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Application
Immunofluorescence of XBP-1 in HepG2 cells with XBP-1 antibody at 20 μg/ml.
Application
Immunocytochemistry of XBP-1 in HepG2 cells with XBP-1 antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | XBP-1 antibody can be used for the detection of XBP-1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | XBP-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | XBP-1 Antibody is supplied in PBS containing 0.02% sodium azide. |
