Application
Western Blot at 1:1000 dilution + human fetal liver lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis of XDH Antibody in mouse lung tissue lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with XDH Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | XDH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Xanthine dehydrogenase/oxidase, Xanthine dehydrogenase, XD, Xanthine oxidase, XO, Xanthine oxidoreductase, XOR, XDH, XDHA |
| Product description | XDH belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The enzyme is a homodimer. This protein can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism.1) Ross,C.J., et.al., Nat. Genet. 41 (12), 1345-1349 (2009) |
| Immunogen | This XDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-234 amino acids from the N-terminal region of human XDH. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | XDH |
|---|---|
| Alternative Names | Xanthine dehydrogenase/oxidase Xanthine dehydrogenase XD Xanthine oxidase XO Xanthine oxidoreductase XOR XDH XDHA |
| Molecular Weight(MW) | 146 kDa |
Application
Western Blot at 1:1000 dilution + human fetal liver lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis of XDH Antibody in mouse lung tissue lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with XDH Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
