Application
Western blot analysis of XIAP in human kidney lysate with XIAP antibody at (A) 0.5, (B) 1 , and (C) 2 μg/mL, respectively.Application
Immunofluorescence of XIAP in Human Kidney tissue with XIAP antibody at 10 μg/mL.Application
Immunohistochemical staining of human kidney tissue using XIAP antibody at 2 μg/mL.| Product Name | XIAP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | XIAP Antibody: API3, ILP1, MIHA, XLP2, BIRC4, IAP-3, hIAP3, hIAP-3, API3, IAP3, E3 ubiquitin-protein ligase XIAP, Baculoviral IAP repeat-containing protein 4, ILP |
| Product description | XIAP Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. The X-chromosome linked inhibitor of apoptosis (XIAP) contains 3 baculoviral IAP repeat (BIR) motifs that are essential and sufficient for the binding and inhibition of caspases-3, -7, and -9. Upregulation of XIAP expression can protect cells from apoptosis induced by low level radiation; conversely, decreased XIAP expression by antisense targeting resulted in increased cell death following low level radiation. Two negative regulators, termed XAF-1 and Smac, can bind and inhibit XIAP activity.1) Schimmer AD. Inhibitor of apoptosis proteins: translating basic knowledge into clinical practice. Cancer Res. 2004; 64:7183-90. |
| Immunogen | XIAP antibody was raised against a synthetic peptide corresponding to 13 amino acids at the C-terminus of human XIAP. The immunogen is located within amino acids 420 - 470 of XIAP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| XIAP antibody can be used for the detection of XIAP by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | XIAP |
|---|---|
| Alternative Names | XIAP Antibody: API3 ILP1 MIHA XLP2 BIRC4 IAP-3 hIAP3 hIAP-3 API3 IAP3 E3 ubiquitin-protein ligase XIAP Baculoviral IAP repeat-containing protein 4 ILP |
| Molecular Weight(MW) | Predicted: 55 kDa Observed: 53 kDa |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
Application
Western blot analysis of XIAP in human kidney lysate with XIAP antibody at (A) 0.5, (B) 1 , and (C) 2 μg/mL, respectively.
Application
Immunofluorescence of XIAP in Human Kidney tissue with XIAP antibody at 10 μg/mL.
Application
Immunohistochemical staining of human kidney tissue using XIAP antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1324 - Human Kidney Tissue Lysate |
|---|---|
| Application Notes | XIAP antibody can be used for the detection of XIAP by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | XIAP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | XIAP Antibody is supplied in PBS containing 0.02% sodium azide. |
