Application
Western Blot at 1:2000 dilution + human liver lysate Lysates/proteins at 20 ug per lane.Application
Antibody is used in Western blot to detect XLKD1 in mouse liver tissue lysate.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis in Y79 cell line lysates (35ug/lane).Application
Western blot analysis of XLKD1 using rabbit polyclonal XLKD1 Antibody (C61) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the XLKD1 gene.| Product Name | XLKD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Lymphatic vessel endothelial hyaluronic acid receptor 1, LYVE-1, Cell surface retention sequence-binding protein 1, CRSBP-1, Extracellular link domain-containing protein 1, Hyaluronic acid receptor, LYVE1, CRSBP1, HAR, XLKD1 |
| Product description | One of the key groups of molecules regulating leukocyte and tumour cell migration is the glycosaminoglycan hyaluronan (HA). In inflammation, the exit of leukocytes across vascular endothelium to the underlying tissues involves interactions with cell surface lectin-like receptors on the leukocytes that bind HA on the lumenal surface of the endothelium. During normal tissue homeostasis and after tissue injury, HA is mobilized from these sites through lymphatic vessels to the lymph nodes where it is degraded before entering the circulation for rapid uptake by the liver. Lymphatic vessel endothelial hyaluronan receptor (LYVE)-1 is a major receptor for HA on the lymph vessel wall. LYVE-1 is expressed primarily on lymphatic vessel endothelium and is likely to be involved in regulating the traffic of leucocytes and tumour cells to lymph nodes.1) Jackson, D.G., Trends Cardiovasc. Med. 13(1):1-7 (2003). |
| Immunogen | This XLKD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-77 amino acids from the N-terminal region of human XLKD1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | LYVE1 |
|---|---|
| Alternative Names | Lymphatic vessel endothelial hyaluronic acid receptor 1 LYVE-1 Cell surface retention sequence-binding protein 1 CRSBP-1 Extracellular link domain-containing protein 1 Hyaluronic acid receptor LYVE1 CRSBP1 HAR XLKD1 |
| Molecular Weight(MW) | 35 kDa |
Application
Western Blot at 1:2000 dilution + human liver lysate Lysates/proteins at 20 ug per lane.
Application
Antibody is used in Western blot to detect XLKD1 in mouse liver tissue lysate.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis in Y79 cell line lysates (35ug/lane).
Application
Western blot analysis of XLKD1 using rabbit polyclonal XLKD1 Antibody (C61) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the XLKD1 gene.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
