Application
Western blot analysis of XPA Antibody in NCI-H460 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with XPA Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | XPA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNA repair protein complementing XP-A cells, Xeroderma pigmentosum group A-complementing protein, XPA, XPAC |
| Product description | XPA is involved in DNA excision repair. It initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. This protein is required for UV-induced CHK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation.1) States J.C., et.al., Hum. Mutat. 12:103-113(1998). |
| Immunogen | This XPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 221-250 amino acids from the C-terminal region of human XPA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | XPA |
|---|---|
| Alternative Names | DNA repair protein complementing XP-A cells Xeroderma pigmentosum group A-complementing protein XPA XPAC |
| Molecular Weight(MW) | 31 kDa |
Application
Western blot analysis of XPA Antibody in NCI-H460 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with XPA Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
