Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | XYLT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Xylosyltransferase 1, Peptide O-xylosyltransferase 1, Xylosyltransferase I, XT-I, XylT-I, XYLT1, XT1 |
| Product description | This locus encodes a xylosyltransferase enzyme. The encoded protein catalyzes transfer of UDP-xylose to serine residues of an acceptor protein substrate. This transfer reaction is necessary for biosynthesis of glycosaminoglycan chains. Mutations in this gene have been associated with increased severity of pseudoxanthoma elasticum.1) Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) : |
| Immunogen | This XYLT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-155 amino acids from the N-terminal region of human XYLT1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | XYLT1 |
|---|---|
| Alternative Names | Xylosyltransferase 1 Peptide O-xylosyltransferase 1 Xylosyltransferase I XT-I XylT-I XYLT1 XT1 |
| Molecular Weight(MW) | 108 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
