Application
Western blot analysis of extracts from COS7 tissue using YAP (Phospho-Ser127) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | YAP (phospho Ser127) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | YAP65, YAP1 |
| Product description | Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact.1) Sorrentino G, et al. (2014) Nat Cell Biol 16, 357-66 |
| Immunogen | YAP (phospho Ser127) antibody was raised against a peptide sequence around phosphorylation site of serine127 (A-H-S (p)-S-P) derived from Human YAP . |
| Modification | p-Ser127 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | YAP1 |
|---|---|
| Alternative Names | YAP65 YAP1 |
| Molecular Weight(MW) | 65 kDa |
| Tissue Specificity | The antibody detects endogenous level of YAP only when phosphorylated at serine 127. |
| Entrez Gene | 10413 |
|---|---|
| Protein Accession | NP_001123617.1 |
Application
Western blot analysis of extracts from COS7 tissue using YAP (Phospho-Ser127) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
