Application
Western blot analysis of YBX2 in human testis tissue lysate with YBX2 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of YBX2 in human testis tissue with YBX2 antibody at 20 μg/mL.Application
Immunohistochemistry of YBX2 in human testis tissue with YBX2 antibody at 10 μg/mL.| Product Name | YBX2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | YBX2 Antibody: DBPC, MSY2, CSDA3, CONTRIN, Y-box-binding protein 2, Contrin, Dbpc |
| Product description | YBX2 Antibody: Germ cell specific Y-box binding protein (YBX2), a germ-cell-specific member of the Y-box family of DNA-/RNA-binding proteins, is a major constituent of messenger ribonucleoprotein particles (mRNPs) and involved in the regulation of the stability and/or translation of germ cell mRNAs. It is proposed to function as a coactivator of transcription in the nucleus and to stabilize and store maternal and paternal mRNAs in the cytoplasm. YBX2 binds to the Y-box consensus promoter element and is expressed in oocytes and testicular germ cells in the stage of spermatogonia to spermatocyte. Recent studies show that deletion of YBX2 leads to the disruption of both spermatogenesis and oogenesis.1) Kohno Y, Matsuki Y, Tanimoto A, et al. Expression of Y-box-binding protein dbpC/contrin, a potentially new cancer/testis antigen. Br. J. Cancer2006; 94:710-6. |
| Immunogen | YBX2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy end of human YBX2. The immunogen is located within amino acids 300 - 350 of YBX2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| YBX2 antibody can be used for detection of YBX2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | YBX2 |
|---|---|
| Alternative Names | YBX2 Antibody: DBPC MSY2 CSDA3 CONTRIN Y-box-binding protein 2 Contrin Dbpc |
| Sequence Similarities | 2) Cat. No. 11-701 - Human Testis Tissue Slide |
Application
Western blot analysis of YBX2 in human testis tissue lysate with YBX2 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of YBX2 in human testis tissue with YBX2 antibody at 20 μg/mL.
Application
Immunohistochemistry of YBX2 in human testis tissue with YBX2 antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1313 - Human Testis Tissue Lysate |
|---|---|
| Application Notes | YBX2 antibody can be used for detection of YBX2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | YBX2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | YBX2 Antibody is supplied in PBS containing 0.02% sodium azide. |
