Application
Antibody used in IHC on Human kidney.Application
Antibody used in WB on Human SW620 cells at 0.2-1 ug/ml.| Product Name | YWHAQ Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | YWHAQ, 1C5, HS1, 14-3-3 |
| Product description | YWHAQ belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis.This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.1) Chen, C.Y., (2006) Biochem. Biophys. Res. Commun. 342 (4), 1203-1210. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human YWHAQ. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| YWHAQ antibody can be used for detection of YWHAQ by ELISA at 1:312500. YWHAQ antibody can be used for detection of YWHAQ by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | YWHAQ |
|---|---|
| Alternative Names | YWHAQ 1C5 HS1 14-3-3 |
| Molecular Weight(MW) | 28 kDa |
Application
Antibody used in IHC on Human kidney.
Application
Antibody used in WB on Human SW620 cells at 0.2-1 ug/ml.| Positive Control | 1) SW620 Cell Lysate using YWHAQ |
|---|---|
| Application Notes | YWHAQ antibody can be used for detection of YWHAQ by ELISA at 1:312500. YWHAQ antibody can be used for detection of YWHAQ by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store YWHAQ antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
